Flameless atomic absorption spectrophotometry of selenium in whole blood.
نویسندگان
چکیده
In this method of analysis for selenium in whole blood by flameless atomic absorption spectrophotometry, 1 mL of sample is first digested with perchloric and nitric acids. After reduction and neutralization, the sample is reacted with 2,3-diaminonaphthalene, and the product is extracted into toluene. Twenty microliters of the extract is injected into the graphite furnace of a flameless atomic absorption spectrophotometer, along with 20 microliters of a 1.0 g/L cupric nitrate solution. Blood-based standards are used to establish the standard curve. The amount of selenium required to give an absorbance of 0.0044 is 5.3 micrograms/L. Precision is good, recovery excellent. The extract is stable for 24 h.
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 28 7 شماره
صفحات -
تاریخ انتشار 1982